Rat IgG IgA IgM (H&L) Antibody Peroxidase Conjugated

SKU: orb347791

Description

Rat IgG IgA IgM (H&L) antibody (Peroxidase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:100,000, IHC: 1:500-1:1,500, WB: 1:500-1:3,000
Reactivity	Rat
Application Notes	Anti-Rat IgG IgA IgM Antibody Peroxidase Conjugated has been tested by ELISA and is suitable for immunoassays where reactivity to different classes of rat immunoglobulins is desired. Antibody has been tested by ELISA, western blot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Rat IgG, IgA and IgM whole molecules
Purity	Anti-Rat IgG IgA IgM (H&L) Antibody, Peroxidase Conjugated, was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Thimerosal
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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goat anti-Rat IgG IgA IgM Antibody peroxidase conjugation, Goat Anti-rat IgGAM HRP conjugated antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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