Rat IgG2b Isotype Control FITC Antibody

SKU: orb623583

Description

Rat monoclonal isotype control against Rat IgG2b conjugated to FITC

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Tested Applications

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

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Antibody Type	Secondary Antibody
Clonality	Monoclonal
Isotype	Rat IgG2b
Clone No.	RTG2B1-2
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using rat IgG2b Isotype control (RTG2B1-2) FITC antibody (concentration in sample 9 μg/ml).

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Protocol Information

Flow Cytometry

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