Rat IgM (mu) Antibody

SKU: orb750729

This product can be custom made

Description

Rat IgM (mu)

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Rat
Application Notes	Anti-Rat IgM antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Antiserum
Immunogen	Rat IgM mu heavy chain
Purity	This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Rat IgM and Rat Serum. No reaction was observed against Rat IgG F(ab’)2 or Rat IgG F(c).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	90 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Rat IgM (mu) Antibody, rabbit anti Rat IgM mu

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Complement regulators Crry, CD55 and CD59 change in placenta and kidney cortex in RUPP compared to Sham animals. B. Immunohistochemistry was conducted as described in Materials and Methods, and staining graded by a blinded observer from 0–3, negative to strongly positive. Representative images at 200X magnification are provided.

IgM deposition is significantly increased in placenta (A) and kidney (B) in RUPP compared to Sham animals. Immunohistochemistry was conducted as described in Materials and Methods, and staining graded by a blinded observer from 0–3, negative to strongly positive. Representative images at 200X magnification are provided.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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