Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

SKU: orb3002125

Description

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Images & Validation

−

Application Notes

Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.5 kDa (IL23A) and 35.9 kDa (IL12B). Subunit IL-23A is fused with a polyhistidine tag at the C-terminus and subunit IL-12B contains no tag. The protein migrates as 23-25 kDa and 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

−

Expression System	HEK293
Target	IL23A & IL12B
Reactivity	Rat
Tag	C-10×His \| Native
Molecular Weight	21.5 kDa & 35.9 kDa
Expression Region	Val 22 - Ala 196 & Met 23 - Ser 335
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

−

Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

IL23A & IL12B

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols