Rat IL12A protein

SKU: orb707323

Description

Recombinant Rat IL-12 Protein is produced by mammalian expression system and the target gene encoding Met23-Ser335 (p40) & Arg23-Ser215 (p35) is expressed with a 6His tag at the C-terminus.

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Rat
Protein Sequence	MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VHRNTDLKFN IKSSSSSPES RAVTCGRASL SAEKVTLNQR DYEKYSVACQ EDVTCPTAEE TLPIELVVEA QQQNKYENYS TSFFIRDIIK PDPPKNLQVK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKTK ETEEECNQKG AFLVEKTSAE VQCKGANICV QAQDRYYNSS CSKWTCVPCR GRSHHHHHH& RVIPVSGPAK CLNQSQNLLK TTDDMVRTAR EKLKHYSCTA GDIDHEDITR DKTSTLEACL PLELHKNESC LATKETSSII RGSCLPPQKT SLMMTLCLGS IYEDLKMYQS EFQAINAALQ SHNHQQITLD RNMLMAIDEL MRSLNHSGET LHQKAPMGEA DPYRVKMKLC ILLHAFSTRV MTINRVMNYL SSSHHHHHH
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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NKSF1; NKSF2; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1; Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2

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Quick Database Links

UniProt

Q9R278, Q9R103

NCBI Gene ID

64546, 84405

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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