Rat Integrin Alpha 2 (ITGa2) ELISA Kit

SKU: orb780747

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2(ITGa2).

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ITGa2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ITGa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ITGa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ITGa2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Rat
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.113 ng/mL
Concentration	20 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

CD49b ELISA Kit, BR ELISA Kit, GPIa ELISA Kit, VLA-2 ELISA Kit, VLAA2 ELISA Kit, Platelet membrane glycoprotein Ia ELISA Kit, Alpha 2 Subunit Of VLA-2 Receptor ELISA Kit, Very Late Activation Proteins 2 Receptor ELISA Kit, Collagen receptor ELISA Kit, VLA-2 alpha ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols