Rat Lipase, Pancreatic (PL) ELISA Kit

SKU: orb780181

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Pancreatic(PL).

Research Area

Cancer Biology, Gastroenterology & Hepatology, Infectious Disease & Virology, Metabolism Research, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.79-50 ng/mL
Sensitivity	0.36 ng/mL
Concentration	50 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PNLIP ELISA Kit, PTL ELISA Kit, Pancreatic Lipase ELISA Kit, Pancreatic triacylglycerol lipase ELISA Kit

Quality Guarantee

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Quick Database Links

UniProt

P27657

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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