Rat Macrophage Derived Chemokine (MDC) ELISA Kit

SKU: orb776410

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine(MDC).

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MDC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MDC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MDC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	23.44-1500 pg/mL
Sensitivity	8.72 pg/mL
Concentration	1500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CCL22 ELISA Kit, ABCD1 ELISA Kit, DC/B-CK ELISA Kit, SCYA22 ELISA Kit, STCP1 ELISA Kit, Chemokine C-C-Motif Ligand 22 ELISA Kit, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22 ELISA Kit, Stimulated T-Cell Chemotactic Protein 1 ELISA Kit

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Rat Macrophage Derived Chemokine (MDC) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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