Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU: orb776409

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	31 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MIP1 Beta,CCL4 ELISA Kit, ACT2 ELISA Kit, G-26 ELISA Kit, LAG1 ELISA Kit, MIP1-B ELISA Kit, SCYA4 ELISA Kit, Chemokine C-C-Motif Ligand 4 ELISA Kit, Small Inducible Cytokine A4 ELISA Kit, Homologous To Mouse Mip-1b ELISA Kit

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Rat Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

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UniProt

P50230

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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