Rat Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) ELISA Kit

SKU: orb780890

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 9(PCSK9).

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Tested Applications

ELISA

Application Notes

standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCSK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCSK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCSK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCSK9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ng/mL
Sensitivity	1.15 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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FH3 ELISA Kit, HCHOLA3 ELISA Kit, NARC1 ELISA Kit, Hypercholesterolemia,Autosomal Dominant 3 ELISA Kit, Neural apoptosis-regulated convertase 1 ELISA Kit, Proprotein convertase 9 ELISA Kit, Subtilisin/kexin-like protease PC9 ELISA Kit

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Rat Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) ELISA Kit

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UniProt

P59996

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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