Rat Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit

SKU: orb778584

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin E Synthase, Microsomal(PTGES).

Research Area

Infectious Disease & Virology, Metabolism Research, Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTGES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTGES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTGES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTGES in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.113 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MGST1L1 ELISA Kit, MGST-IV ELISA Kit, MGST1-L1 ELISA Kit, PGES ELISA Kit, PIG12 ELISA Kit, PP102 ELISA Kit, PP1294 ELISA Kit, TP53I12 ELISA Kit, PGE Synthase ELISA Kit, Tumor Protein p53 Inducible Protein 12 ELISA Kit, Glutathione S-Transferase 1-Like 1 ELISA Kit, MGST1-Like 1 ELISA Kit

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Rat Prostaglandin E Synthase, Microsomal (PTGES) ELISA Kit

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UniProt

Q9JHF3

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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