Rat testis expressed 101 (TEX101) ELISA Kit

SKU: orb866865

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Description

Sandwich Rat TEX101 ELISA Kit

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TEX101. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TEX101. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TEX101, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TEX101 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.63-40 ng/mL
Sensitivity	0.262 ng/mL
Concentration	40 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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Cell surface receptor NYD SP8 ELISA Kit, GTPR867 ELISA Kit, NYD SP8 ELISA Kit, PRO1884 ELISA Kit, SGRG ELISA Kit, TES101RP ELISA Kit, testis expressed 101 ELISA Kit, Testis expressed protein 101 ELISA Kit, TEX101 ELISA Kit

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Rat testis expressed 101 (TEX101) ELISA Kit

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UniProt

Q924B5

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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