Rat Triiodothyronine (T3) ELISA Kit

SKU: orb782509

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Triiodothyronine(T3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Triiodothyronine(T3).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat T3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	156.25-10000 pg/mL
Sensitivity	50.3 pg/mL
Concentration	10000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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3,3',5-Triiodo-L-Thyronine ELISA Kit, Liothyronine ELISA Kit, Cytomel ELISA Kit, Tertroxin ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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