Rat Troponin C Type 1, Slow (TNNC1) ELISA Kit

SKU: orb782710

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin C Type 1, Slow(TNNC1).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNNC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNNC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNNC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNNC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.59 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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TNC ELISA Kit, TroponinC, Cardiac ELISA Kit, Troponin C, slow skeletal and cardiac muscles ELISA Kit

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Rat Troponin C Type 1, Slow (TNNC1) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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