Recombinant Bcl-2 Antibody

SKU: orb2637166

Description

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Research Area

Cell Biology

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	rBCL2/6418
Immunogen	Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human HDLM-2 cell lysate with recombinant Bcl-2 antibody. Predicted molecular weight ~26 kDa.

IHC staining of FFPE human tonsil tissue with recombinant Bcl-2 antibody (clone rBCL2/6418). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl-2 antibody (rBCL2/6418) as confirmation of integrity and purity.

Quick Database Links

UniProt

P10415

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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