Recombinant CD3 epsilon Antibody

SKU: orb2635693

Description

Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains: epsilon, gamma, delta and zeta. The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR). CD3 antigen appears at all stages of T cell development which makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD3 epsilon antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	C3e/4653R
Immunogen	A portion of amino acids 100-200 was used as the immunogen for the recombinant CD3 epsilon antibody.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R). Predicted molecular weight ~23 kDa.

IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone CD3e/4653R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant CD3 epsilon antibody (clone C3e/4653R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P07766

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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