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Human APRIL
SKU: orb2994084
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human APRIL -Flag-His(Cat#orb2994084) at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc(Cat#orb2994273).The ED50 of Cynomolgus BCMA-Fc(Cat#orb2994273) is 4.23 ng/ml.. BLI: Loaded Cynomolgus BCMA-Fc(Cat#orb2994273) on Protein A Biosensor, can bind Human APRIL-His(Cat#orb2994084) with an affinity constant of 0.005 nM as determined in BLI assay. |
| Tag | N-Flag-His |
| Molecular Weight | Predicted: 50 KDa. Observed: 60 KDa, reducing conditions |
| Expression Region | Lys112-Leu250 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 1M NaCl, pH 8.3. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13
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Human APRIL (orb2994084)
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