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Human CD40L
SKU: orb2994532
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human CD40L mAb(Cat. No.:orb2993151) at 5 μg/mL (100 μL/well) can bind Human CD40L (Cat. No.:orb2994532). The EC50 of Human CD40L (Cat. No.:orb2994532) is ≤10 ng/mL.（QC Verified）. BLI: Loaded Human CD40-mFc(Cat#orb2994039) on AMC Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 10.2 nM as determined in BLI assay. Loaded Human CD40-Fc(Cat#orb2994010) on Protein A Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 3.29 nM as determined in BLI assay. Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 0.02 nM as determined in BLI assay. |
| Tag | Tag free |
| Molecular Weight | Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions |
| Expression Region | Met113-Leu261 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
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Human CD40L (orb2994532)
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