Recombinant Human Complement factor D (CFD) (Active)

SKU: orb2986240

Active

Description

This Recombinant Human Complement factor D(CFD) (Active) spans the amino acid sequence from region 26-253aa. Purity: Greater than 95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Key Properties

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Source	Mammalian cell
Biological Origin	Homo sapiens (Human)
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/mL can bind Anti-CFD recombinant antibody. The EC50 is 31.86-39.21 ng/mL.
Tag	C-terminal 10xHis-tagged
Expression Region	26-253aa
Protein Length	Full Length of Mature Protein
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.
MW	25.9 kDa
Purity	Greater than 95% as determined by SDS-PAGE.
Protein Sequence	ILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLA

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Complement factor D; EC:3.4.21.46; Adipsin; C3 convertase activator; Properdin factor D; CFD;DF, PFD

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Recombinant Human Complement factor D (CFD) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized Human CFD at 2 μg/ml can bind CFD Antibody (orb3053414). The EC50 is 31.86-39.21 ng/mL.

Quick Database Links

UniProt

P00746

UniProt Details

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No UniProt data available

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