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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 x103 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rHuEpigen as determined by LAL method. |
| MW | Approximately 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EPG, Epithelial Mitogen
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WB analysis of orb1906270.
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Recombinant Human Epigen (orb1906270)
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