Recombinant Human Fibroblast Growth Factor 21

SKU: orb1906282

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Description

Recombinant Human Fibroblast Growth Factor 21

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 x103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.
Endotoxins	Less than 1 EU/ug of rHuFGF-21 as determined by LAL method.
MW	Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Protein Sequence	HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

Storage & Handling

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Form/Appearance	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Human FGF21 Protein, hFc Tag [orb1290908]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Human FGF21 Protein, N-Fc [orb2963293]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
MW:
47.40 kDa
Sizes Available: 1 mg, 50 μg, 100 μg

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Recombinant Human Fibroblast Growth Factor 21

WB analysis of orb1906282.

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UniProt

Q9NSA1

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