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Recombinant human IL-18 protein
Description
Research Area
Images & Validation
−Key Properties
−| Expression System | E.coli |
|---|---|
| Target | Interleukin-18 |
| Tag | Tag Free |
| Molecular Weight | 18.2 kDa |
| Protein Sequence | 37-193/193 |
| Purification | AC |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxins | Not analyzed |
Storage & Handling
−| Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 20mM Tris-HCl (pH8.0) with 2M Urea. |
| Concentration | >1mg/ml |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL18 Protein, hFc Tag [orb1173723]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide.The apparent molecular mass of IL18-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgRecombinant Mouse IL-18BPd Protein [orb3002275]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 45.1 KDa. Observed: 60-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-18 Protein [orb3002413]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 18.2 KDa. Observed: 17 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μg

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The purity of the protein is greater than 80% as determined by reducing SDS-PAGE.
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UniProt Details
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Protocol Information
Recombinant human IL-18 protein (orb1974371)
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