Human IL-23A & mouse IL-12B Heterodimer

SKU: orb2993931

Description

Recombinant Chimeric Human&Mouse Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Arg20-Pro189&Met23-Ser335 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human&Mouse
Biological Activity	ELISA: Immobilized Human IL-23R-Fc(Cat#orb2994313) at 10μg/ml (100 μl/well) can bind Human IL-23A & mouse IL-12B Heterodimer-His(Cat#orb2993931). The ED50 of Human IL-23A & mouse IL-12B Heterodimer-His(Cat#orb2993931) is 0.48 ug/ml. Immobilized Human IL-23A & mouse IL-12B Heterodimer-His(Cat#orb2993931) at 2μg/ml (100 μl/well) can bind Anti-Human IL23-P19 mAb(Cat#orb2993116).The ED50 of Anti-Human IL23-P19 mAb(Cat#orb2993116) is 7.47 ng/ml.. BLI: Loaded Human IL-23R-Fc(Cat#orb2994313) on Protein A Biosensor, can bind Human IL-23A & mouse IL-12B Heterodimer-His(Cat#orb2993931) with an affinity constant of 9.48 nM as determined in BLI assay. Loaded Anti-Human IL23-P19 mAb-Fc(Cat#orb2993116) on Protein A Biosensor, can bind Human IL-23A & mouse IL-12B Heterodimer-His(Cat#orb2993931) with an affinity constant of -3 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 357.68 ng/ml.
Tag	C-6His
Molecular Weight	Predicted: 56.9 KDa. Observed: 65-80 KDa, reducing conditions
Expression Region	Arg20-Pro189&Met23-Ser335
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

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UniProt

Q9NPF7, P43432

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No UniProt data available

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