Recombinant Human LIGHT Protein

SKU: orb2994330

Description

Recombinant Human TNF Ligand Superfamily Member 14 is produced by our Mammalian expression system and the target gene encoding Leu83-Val240 is expressed with a 6His tag at the N-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Recombinant Human LIGHT (N-6His)(Cat#orb2994330) at 5μg/ml (100 μl/well) can bind Recombinant Rhesus Macaque HVEM (C-Fc)(Cat#orb2994821).The ED50 of Recombinant Rhesus Macaque HVEM (C-Fc)(Cat#orb2994821) is 17.51 ng/ml. (Regularly tested). BLI: Loaded Mouse LTBR-Fc(Cat#orb2993964) on Protein A Biosensor, can bind Human LIGHT-His(Cat#orb2994330) with an affinity constant of 0.81 nM as determined in BLI assay. (Regularly tested)
Tag	N-6His
Molecular Weight	Predicted: 18.2 KDa. Observed: 18-24 KDa, reducing conditions
Expression Region	Leu83-Val240
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; TNFSF14; HVEM-L; LIGHT

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UniProt

AAC39563.1

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No UniProt data available

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