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Human Nectin-2
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human PVRIG-mFc(Cat#orb2993726) at 10μg/ml (100 μl/well) can bind Human Nectin-2-His(Cat#orb2995078) The ED50 of Human Nectin-2-His(Cat#orb2995078) is 3.72 ug/ml. Immobilized Human PVRIG-Fc(Cat#orb2993712) at 10μg/ml (100 μl/well) can bind Human Nectin-2-His(Cat#orb2995078) The ED50 of Human Nectin-2-His(Cat#orb2995078) is 2.914 ug/ml. Immobilized Human DNAM-1-Fc (Cat#orb2994153) at 2μg/ml (100 μl/well) can bind Human Nectin-2-His (Cat#orb2995078). The ED50 of Recombinant Human Nectin-2-His (Cat#orb2995078) is 0.3-1.5 ug/ml. |
| Tag | C-6His |
| Molecular Weight | Predicted: 36.58 KDa. Observed: 50 KDa, reducing conditions |
| Expression Region | Gln32-Leu360 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Polyclonal
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100 μgBiotinylated Human Nectin-2 [orb2993711]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 38.4 KDa. Observed: 45-60 KDa, reducing conditions
20 μg, 100 μgHuman Nectin-2 [orb2993695]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.6 KDa. Observed: 80-90 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman Nectin-2 [orb2993053]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.5 KDa. Observed: 75-85 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgMouse Nectin-2 [orb2993054]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 61.5 KDa. Observed: 65-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Human Nectin-2 (orb2995078)
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