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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Mouse OX40L-His(Cat#orb2994337) at 10μg/ml (100 μl/well) can bind Human OX40-Fc(Cat#orb2994421).The ED50 of Human OX40-Fc(Cat#orb2994421)is 0.10 ug/ml.. BLI: Loaded Biotinylated Mouse OX40L-His(Cat#orb2994337) on AR2G Biosensor, can bind Human OX40-Fc(Cat#orb2994421) with an affinity constant of 907 nM as determined in BLI assay. |
| Tag | N-Fc |
| Molecular Weight | Predicted: 46.3 KDa. Observed: 40-60 KDa, reducing conditions |
| Expression Region | Leu29-Ala216 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor receptor superfamily member 4;TNFRSF4;OX40;CD134;Txgp1
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Recombinant Human OX40 Protein (orb2994421)
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