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Human PD-1
Description
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human PD-1 mAb(Cat#GMP-A085) at 2μg/ml (100 μl/well) can bind Human PD-1-mFc(Cat#orb2994924). The ED50 of Human PD-1-mFc(Cat#orb2994924) is 12.53 ng/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on AHC Biosensor, can bind Human PD-1-His(Cat#orb2994924) with an affinity constant of -3 nM as determined in BLI assay. |
| Tag | C-mFc |
| Molecular Weight | Predicted: 42 KDa. Observed: 60 KDa, reducing conditions |
| Expression Region | Pro21-Gln167 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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0.16-10ng/mL
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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Unconjugated
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Human PD-1 (orb2994924)
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