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Human PD-1

SKU: orb2994924

Description

Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Anti-Human PD-1 mAb(Cat#GMP-A085) at 2μg/ml (100 μl/well) can bind Human PD-1-mFc(Cat#orb2994924). The ED50 of Human PD-1-mFc(Cat#orb2994924) is 12.53 ng/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on AHC Biosensor, can bind Human PD-1-His(Cat#orb2994924) with an affinity constant of -3 nM as determined in BLI assay.
TagC-mFc
Molecular WeightPredicted: 42 KDa. Observed: 60 KDa, reducing conditions
Expression RegionPro21-Gln167
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
DisclaimerFor research use only

Alternative Names

Programmed cell death protein 1; hPD-1; PDCD1; CD279

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Human PD-1 (orb2994924)

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Available Sizes

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10 μg
¥ 1,950.00
50 μg
¥ 4,160.00
500 μg
¥ 22,620.00
1 mg
¥ 32,110.00
DispatchUsually dispatched within 5-10 working days
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