Recombinant human Semaphorin 4D / CD100 protein, C-His-Avi (HEK293)

SKU: orb1516336

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Description

Recombinant human Semaphorin 4D / CD100 protein, C-His-Avi (HEK293), with a concentration of >0.5 mg/ml, expressed in HEK293 cell, and a purity of >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC. It was purified by AC, and has a C-His-Avi tag. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Images & Validation

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Key Properties

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Expression System	HEK293 cell
Target	Semaphorin-4D
Tag	C-His-Avi
Molecular Weight	Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Protein Sequence	22-734/862
Purification	AC
Purity	>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Endotoxins	<1.0 EU/μg as determined by LAL

Storage & Handling

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Storage	Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
Concentration	>0.5 mg/ml
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Semaphorin-4D; Semaphorin4D; A8 antibody BB 18; BB18; CD 100; CD100; CD100 antigen; Coll 4; COLL4; Collapsin 4; Collapsin4; GR3; Human semaphorin CD100 mRNA complete cds; Leukocyte activation antigen CD100; M sema G; MSEMA; SEM4D_HUMAN; Sema 4d; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ; Sema H; SEMA J; Semacl 2; Semacl2; SemaH; SEMAJ; Semaphorin 4D; Semaphorin 4D precursor; Semaphorin C like 2; Semaphorin H; Semaphorin J; SemaphorinJ; Semcl 2; Semcl2; Recombinant human Semaphorin 4D / CD100 protein, C-His-Avi (HEK293)

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Recombinant human Semaphorin 4D / CD100 protein, C-His-Avi (HEK293)

Human Semaphorin 4D/CD100 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Semaphorin 4D/CD100 is greater than 95% as determined by SEC-HPLC.

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Gene Symbol

Semaphorin-4D

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