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Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor/TNFRSF11A
SKU: orb1906296
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Description
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand. |
| Endotoxins | Less than 0.1 EU/ug of rHusRANK Receptor as determined by LAL method. |
| MW | Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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WB analysis of orb1906296.
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Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor/TNFRSF11A (orb1906296)
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