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Human ST2
SKU: orb2994520
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human ST2-His(Cat#orb2994520) at 5μg/ml (100 μl/well) can bind Human IL-33-His(Cat#C233).The ED50 of Human IL-33-His(Cat#C233) is 8.44 ng/ml.. BLI: Loaded Human IL-33(Cat#orb2995234) on AR2G Biosensor, can bind Human ST2-His(Cat#orb2994520) with an affinity constant of 0.97nM as determined in BLI assay. Loaded Human sST2 Antibody-mFc(Cat#DA014) on AMC Biosensor, can bind Human ST2-His(Cat#orb2994520) with an affinity constant of -3 nM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 36 KDa. Observed: 50-85 KDa, reducing conditions |
| Expression Region | Lys19-Phe328 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-1 receptor-like 1;Protein ST2;DER4; ST2; T1
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Human ST2 (orb2994520)
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