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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 x106 IU/mg. |
| Endotoxins | Less than 1 EU/ug of rHuTSLP as determined by LAL method. |
| MW | Approximately 15.1 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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WB analysis of orb1906317.
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Recombinant Human Thymic Stromal Lymphopoietin (orb1906317)
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