Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2, Variant

SKU: orb1906313

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Description

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2, Variant

Research Area

Cancer Biology; Immunology & Inflammation

Images & Validation

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Item 1 of 1

Key Properties

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg in the presence of actinomycin D.
Endotoxins	Less than 1 EU/ug of rHu TNF-α/TNFSF2, Variant as determined by LAL method.
MW	Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 151 amino acids. Compared with the wild-type, rHuTNF-α Variant has an amino acid sequence (a.a.) deletion from a.a. 1-7, and the following a.a. substitutes Arg8, Lys9, Arg10 and Phe157 which is proven to have more activity and with less inflammatory side effect in vivo.
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Protein Sequence	MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F

Storage & Handling

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Form/Appearance	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

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Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2, Variant

SDS-PAGE gel analysis of orb1906313.

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UniProt

P01375

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