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Recombinant Human Vascular Endothelial Growth Factor 165, Yeast-derived
SKU: orb1906271
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Description
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Key Properties
−| Source | Yeast |
|---|---|
| Expression System | Yeast |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0 ng/ml. |
| Molecular Weight | Theoretically as a disulfide-linked homodimeric protein, the product consists of two 166 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least three bands with molecular weights ranging from 20-31 kDa in SDS-PAGE under reducing conditions. |
| Protein Sequence | MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rHuVEGF165 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20. |
| Disclaimer | For research use only |
Alternative Names
−Vascular Endothelial Growth Factor Isoform 165
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Recombinant Human Vascular Endothelial Growth Factor 165, Yeast-derived (orb1906271)
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