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Human VEGFR2
Description
Images & Validation
−
Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human VEGFR2-His(Cat#orb2994764) at 1μg/ml (100 μl/well)can bind Anti-Human VEGFR2 mAb(Cat#orb2993814).The ED50 of Anti-Human VEGFR2 mAb(Cat#orb2993814) is 36.55 ng/ml.. BLI: Loaded Anti-Human VEGFR2 mAb-Fc(Cat#orb2993814) on Protein A Biosensor, can bind Human VEGFR2-His(Cat#orb2994764) with an affinity constant of 32.85 pM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 84.3 KDa. Observed: 110-140 KDa, reducing conditions |
| Expression Region | Ala20-Glu764 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 86.1 KDa. Observed: 120-150 KDa, reducing conditions
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 110.4 KDa. Observed: 150-160 KDa, reducing conditions
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Human VEGFR2 (orb2994764)
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