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Human VEGFR2
SKU: orb2994455
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human VEGF121-His (Cat#orb2994931) at 10μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455). The ED50 of Recombinant Human VEGFR2-Fc (Cat#orb2994455) is 1.92 ug/ml. Immobilized Human VEGF 121(Cat#orb2994925) at 2μg/ml (100 μl/well)can bind Human VEGFR2-Fc(Cat#orb2994455) .The ED50 of Human VEGFR2-Fc(Cat#orb2994455) is 13.04 ng/ml. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 110.4 KDa. Observed: 150-160 KDa, reducing conditions |
| Expression Region | Ala20-Glu764 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 15% Maltose, 0.05% Tween 80, pH 9.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1
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Human VEGFR2 (orb2994455)
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