Recombinant IL-33 (Interleukin-33), Human, AF

SKU: orb1179048

Description

Images & Validation

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Tested Applications	ELISA

Key Properties

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Source	Escherichia coli
Biological Activity	Measure by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is <54 ng/mL. Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is < 0.1 ng/mL. The specific activity of recombinant human IL-33 is approximately >4 x107 IU/ mg.
Reactivity	Human
Tag	His-tag at the N-terminus
Protein Sequence	MSITGISPITEYLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET with polyhistidine tag at the C-terminus.
Purity	>98% as determined by SDS-PAGE. Ni-NTA chromatography.
Endotoxins	<0.1 EU per 1 μg of the protein by the LAL method.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Form/Appearance	Lyophilized
Buffer/Preservatives	The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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IL-1 F11, NF-HEV

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Quick Database Links

NCBI Gene ID

90865

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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