Recombinant Mouse CCL3

SKU: orb623233

Description

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is a small cytokine belonging to the CC chemokine family. CCL3/MIP-1 alpha is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

1. Reconstitute each vial of Nigericin with 100 µL DMSO to form the 5 mM stock concentrate. Once reconstituted, it may be aliquoted and stored at ≤ -20°C for 1 year protected from light and thawed no more than twice during that time. 2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 µM working solution for use in treating samples. For example, dilute 1:10 by adding 20 µL stock concentrate to 180 µL diH2O. 3. Use Nigericin at 1-20 µM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 µM, dilute 500 µM working solution 1:50 in samples; e.g. spike 294 µL cell suspension/overlay medium with 6 µL of 500 µM working solution. Typical treatment periods range from 3-24 hours at 37°C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions. 4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1β or IL-18, or through the use of caspase-1 activation assays, such as ICT’s Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit.

Key Properties

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Source	Yeast
MW	7.9 kDa
Protein Sequence	APFGADTPTA CCFSYVARQL SRKIVADYFE TSSQCSKPGV IFQTKKGRQV CANPTEDWVQ EYITDLELNA (70)

Storage & Handling

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Storage	-20°C
Form/Appearance	Lyophilized
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Applications:	ELISA, IHC, WB
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IHC, WB
Reactivity:	Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, SDS-PAGE, WB
Purity:	>90% as determined by SDS-PAGE.
MW:	20.23 kDa

Purity:	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
MW:	Predicted: 10.8 KDa. Observed: 15 KDa, reducing conditions

Purity:	Greater than 90% as determined by SDS-PAGE.
MW:	12.0 kDa
Source:	E.coli

Recombinant Mouse CCL3

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

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Protocol Information

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