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Mouse GITR
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Tag | C-Fc-6His |
| Molecular Weight | Predicted: 42.3 KDa. Observed: 60 KDa, reducing conditions |
| Expression Region | Ser22-His153 |
| Purity | SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 5% Sucrose, 5% Dextran-70,0.05% Tween 80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Human GITR [orb2994045]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 41.1 KDa. Observed: 40-55 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman GITR Protein, mFc-His tag [orb689380]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50-52 kDa after removal of the signal peptide.
Mammalian
100 μg, 50 μg, 10 μgHuman GITR Ligand Protein, mFc-His tag [orb689381]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46-53 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μgMouse GITR Protein, hFc Tag [orb1173691]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.3 kDa after removal of the signal peptide.The apparent molecular mass of mGITR-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μgTnfrsf18 Antibody [orb607823]
ELISA
Human
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide)

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Protocol Information
Mouse GITR (orb2993965)
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