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Mouse TIGIT
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Recombinant Mouse PVR (C-6His) (Cat#orb2992802) on HIS1K Biosensor, can bind Recombinant Mouse TIGIT (C-Fc) (Cat#orb2994212) with an affinity constant of 13.2 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc |
| Molecular Weight | Predicted: 40.1 KDa. Observed: 50-60 KDa, reducing conditions |
| Expression Region | Gly26 - Thr143 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Mouse TIGIT [orb2994425]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 13.3 KDa. Observed: 15-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman TIGIT [orb2994060]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 39.7 KDa. Observed: 40-50 KDa, reducing conditions
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Protocol Information
Mouse TIGIT (orb2994212)
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