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Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg. |
| Endotoxins | Less than 0.1 EU/ug of rRhG-CSF as determined by LAL method. |
| MW | Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL RHSLGIPWAP LSSCPSQALQ LTGCLSQLHS SLFLYQGLLQ ALEGISPELS PTLDTLQLDI ADFATTIWQQ MEDLGMAPAL QPTQGAMPAF TSAFQRRAGG VLVASHLQRF LELAYRVLRH LAQS |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Approximately 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.
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10 μg, 100 μg, 500 μgRecombinant Rhesus Macaque Granulocyte-macrophage colony-stimulating factor protein(GM-CSF) (Active) [orb1650561]
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SDS-PAGE gel analysis of orb1906214.
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Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor (orb1906214)
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