RecombinantMIF,Human

SKU: orb1494795

Description

Macrophage Migration Inhibitory Factor (MIF) is a pleiotropic cytokine, existing as a homotrimer in vivo. MIF was originally identified as a T cell derived factor responsible for the inhibition of macrophage migration. However, recently MIF has received much more attention because of its possible roles in angiogenesis and cancer development. MIF is over-expressed in various cancers, including pancreatic, breast, colon, brain, prostate, skin, and lung. The intratumoral expression of MIF is strongly correlated with angiogenic growth factor expression, such as the expression of Interleukin 8 (IL-8) and Vascular Endothelial Growth Factor (VEGF), and with risk of recurrence after resection. Recombinant human Macrophage Migration Inhibitory Factor (rhMIF) produced in E.coli is a single non-glycosylated polypeptide chain containing 115 amino acids. rhMIF has a molecular mass of 12.5 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Molecular Weight	12.5 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MPMFIVNTN VPRASVPDGF LSELTQQLAQ ATGKPPQYIA VHVVPDQLMA FGGSSEPCAL CSLHSIGKIG GAQNRSYSKL LCGLLAERLR ISPDRVYINY YDMNAANVGW NNSTFA
Purification	> 95% as analyzed by SDS-PAGE and HPLC.
Purity	> 95% as analyzed by SDS-PAGE and HPLC.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Macrophage Migration Inhibitory Factor (rhMIF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhMIF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Macrophage Migration, Inhibitory Factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor

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