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Description
Research Area
Immunology & Inflammation; Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FA, FACS, In vivo |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human Humanized |
| Clonality | Monoclonal |
| Isotype | IgG4 |
| Clone No. | BEEX12224 |
| Source/Expression System | Mammalian Cells |
| Purity | >95% purity as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin | Please contact with the lab for this information. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6
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Quality Guarantee
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Flow-cytometry using APC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD47 monoclonal antibody (Catalog: orb2977687, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD47 monoclonal antibody (Catalog: orb2977687, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD47 monoclonal antibody (Catalog: orb2977687, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Research Grade Anti-Human CD47/MER6 Antibody.
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UniProt Details
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD47/MER6 Human Monoclonal Antibody (orb2977687)
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