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RPL23 Antibody (Center)
SKU: orb1937356
Description
Research Area
Infectious Disease & Virology, Molecular Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Porcine, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 14865 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Quality Guarantee
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All lanes: Anti-RPL23 Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: THP-1 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: mouse brain lysate. Lane 7: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RPL23 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RPL23 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the RPL23 antibody detected the RPL23 protein (arrow).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
RPL23 Antibody (Center) (orb1937356)
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