SAG

SKU: orb1307461

Description

SAG

Research Area

Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	912545-86-9
MW	490.06
Purity	98.88%
Formula	C₂₈H₂₈ClN₃OS
SMILES	CN[C@H]1CC[C@@H](CC1)N(Cc1cccc(c1)-c1ccncc1)C(=O)c1sc2ccccc2c1Cl
Target	Hedgehog/Smoothened,Smo
Solubility	DMSO:252.5 mg/mL (515.24 mM);Ethanol:40 mg/mL (81.62 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.5 mg/mL (11.22 mM)

Bioactivity

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Target IC50	C3H 10T1/2 cells:0.13 μM (EC50)\|Shh Light II cells:8.8 nM (EC50)\|Smo:3 nM (EC50)\|HUVECs:22 μM
In Vivo	METHODS: To explore whether the SHH signaling pathway plays a protective role in anxiety by regulating mitochondrial homeostasis, SAG (10 mg/kg) was intraperitoneally injected into C57BL/6 mice on a high-fat diet every three days for twelve weeks. RESULTS: SHH signaling is neuroprotective in obesity, and SAG alleviates anxiety-like behaviors by reducing mitochondrial breaks. METHODS: To study the effects on developing limbs, SAG (15-20 mg/kg in lactated Ringer's solution) was administered as a single intraperitoneal injection to pregnant C57BL/6J mice at gestational day (GD) 9.25. RESULTS: The most prevalent effect of SAG was the dose-dependent induction of preaxial polydactyly; defects ranged from thumb width to duplication of both digitiform fingers on the preaxial side of the thumb. Read more...
In Vitro	METHODS: Embryonic stem cell ES were treated with SAG (0.5-1.25 µM) for 4-6 days and gene expression levels were measured using RT-qPCR. RESULTS: Expression of Sim1, a post-mitotic V3 marker, was increased in all SAG-treated groups at all concentrations. The group treated with 0.5 μM SAG exhibited significantly higher levels of Nkx2.2 mRNA. Hb9 expression did not change significantly with SAG concentration at the given time points. METHODS: African green monkey kidney fibroblast-like cells, Cos-1, were treated with SAG (5-500 nM), and target protein expression levels were measured by Western Blot. RESULTS: SAG inhibited cross-linking of ER-localized and post-ER forms of Smo-Myc3 to 125I-labeled PA cyclic amines in Cos-1 cells in a dose-dependent manner. cellular levels of Smo-Myc3 were not affected by agonist treatment. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SAG, Smoothened Agonist, Smoothened, Smo, Hedgehog

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Compound Identifiers

PubChem CID

5284330 ↗

Key Properties

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No computed properties available.

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SAG (orb1307461)

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Applications:	ELISA, FC, IHC, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, FC, ICC, IF, IHC, WB
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	WB
Predicted Reactivity:	Human
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Mouse
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.054 ng/mL

Reactivity:	Human
Dynamic Range:	0.16-10 ng/mL
Sensitivity:	0.056 ng/mL

SAG

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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SAG (orb1307461)

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