SCP2 Antibody (Center)

SKU: orb1928378

Description

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC-P, WB
Dilution range	WB - 1:500-2000, IHC-P - 1:100-500, FC - 1:10-50
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Rabbit

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	58994 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

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Flow cytometric analysis of HepG2 cells using SCP2 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SCP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-SCP2 Antibody (Center) at 1:500-2000 dilution. Lane 1: Mouse liver tissue lysate. Lane 2: Rat liver tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P22307

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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