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Description
Research Area
Metabolism Research
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | SDHB/2126 |
| Immunogen | A portion of amino acids 165-273 was used as the immunogen for the SDHB antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot testing of human 1) 293, 2) Jurkat and 3) HepG2 cell lysate using SDHB antibody (clone SDHB/2126).
IHC staining of FFPE human kidney tissue with SDHB antibody (clone SDHB/2126). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SDHB antibody (SDHB/2126) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
SDHB Antibody (orb1151870)
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