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SETDB1 Recombinant Mouse Monoclonal Antibody
SKU: orb1172934
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Description
Research Area
Cell Biology, DNA and RNA, Epigenetics and Nuclear Signaling
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-2000 |
| Reactivity | Human |
| Predicted Reactivity | Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1, Kappa |
| Clone No. | H4A4 |
| Immunogen | Recombinant human KMT1E protein |
| Target | SETDB1 |
| Molecular Weight | 143 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ERG-associated protein with SET domain; ESET; H3 K9 HMTase 4; H3 lysine 9 specific 4; H3-K9-HMTase 4; H3-K9-HMTase4; Histone H3 K9 methyltransferase 4; Histone H3-K9 methyltransferase 4; Histone lysine N methyltransferase; Histone-lysine N-methyltransferase SETDB1; KG1T; KIAA00067; KIAA0067; KMT1E; Lysine N-methyltransferase 1E; MGC90670; mKIAA0067; SET domain bifurcated 1; Set domain protein, bifurcated, 1; SETB; SETB1_HUMAN; SETDB1; Similar to DNA topoisomerase II.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Lysate: 1: HeLa, 2: JurKat, 3: NIH/3T3, 4: COS-7, 5: PC-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 170 kDa, Observed MW: 170 kDa.
Cell line: Ramos, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172934.
Quick Database Links
Gene Symbol
SETDB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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SETDB1 Recombinant Mouse Monoclonal Antibody (orb1172934)
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