Sheep IgG F(ab')2 Antibody Pre-Adsorbed

SKU: orb347917

Description

Sheep IgG F(ab')2 antibody

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Sheep
Application Notes	Anti-Sheep IgG F(ab’)2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Sheep IgG F(ab')2 fragment
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum. No reaction was observed against Sheep IgG F(c). This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but antibodies may cross-react with immunoglobulins from other species.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti Sheep IgG F(ab')2 Antibody, Rabbit Anti-Sheep IgG Fab2 Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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