You have no items in your shopping cart.
Sheep IgG Fc Antibody
SKU: orb347913
Description
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit. |
| Purity | Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
Similar Products
−
Rabbit Sheep IgM (Fc specific), conjugated with Biotin Antibody [orb21728]
Sheep
Rabbit
Polyclonal
Biotin
1 ml
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
ELISA Results of Rabbit Anti-Sheep IgG F(c) Antibody tested against purified Sheep IgG F(c). Each well was coated in duplicate with 1.0 µg of Sheep IgG F(c) (p/n orb346368) [Red Line] and Sheep IgG [Green Line]. The working dilution of Sheep IgG F(c) is 1:45000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).
Quick Database Links
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Sheep IgG Fc Antibody (orb347913)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days