Sheep IgG (H&L) Antibody Texas Red Conjugated

SKU: orb347962

Description

Sheep IgG (H&L) antibody (Texas Red)

Images & Validation

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Item 1 of 1

Tested Applications	FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Sheep
Application Notes	Sheep IgG (H&L) Antibody Texas Red Conjugated antibody has been tested by western blot and are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG
Immunogen	Sheep IgG whole molecule
Purity	Sheep IgG (H&L) Antibody Texas Red Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
Conjugation	Texas Red

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey Anti-Sheep IgG Texas Red Conjugated Antibody, Donkey Anti-Sheep IgG Antibody Texas Red Conjugation

Quality Guarantee

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Sheep IgG (H&L) Antibody Texas Red Conjugated

Western Blot of Donkey anti-Sheep IgG Texas Red Conjugated Antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None.

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence